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Tuesday, September 13, 2005. The DNA double helix can under certain conditions accommodate a third strand in its major groove. Researchers in the UK have now presented a complete set of four variant nucleotides that makes it possible to use this phenomenon in gene regulation and mutagenesis. They tested the binding of a 19-mer of these designer nucleo-tides to a double helix target sequence in comparison with the corresponding triplex-forming oligonucleotide made from natural DNA bases.
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